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07-722 Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) Antibody

Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) Antibody is an antibody against phospho-VEGFR2/Flk-1/KDR (Tyr1054) for use in WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: KDR, VEGFR-2, VEGFR2, VEGFR, CD309, FLK1, EC 2.7.10.1 UniProt Number: P35968
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07-722
100 µg  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Pm, B, Ch, Eq, ZebrafishWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-722
      Trade Name
      • Upstate
      DescriptionAnti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) Antibody
      Alternate Names
      • Kinase insert domain receptor
      • Protein-tyrosine kinase receptor Flk-1
      • kinase insert domain receptor (a type III receptor tyrosine kinase)
      • vascular endothelial growth factor receptor 2
      Background InformationThe Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 (also known as Flk-2) is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines.
      References
      Product Information
      FormatAffinity Purified
      Control
      • CSF-1 treated PAE cell lysate, transfected with CSF/KDR.
      PresentationAffinity purified rabbit polyclonal IgG in PBS with 0.05% NaN3 and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) Antibody is an antibody against phospho-VEGFR2/Flk-1/KDR (Tyr1054) for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenSynthetic peptide encompassing
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes VEGFR2/Flk-1/KDR when phosphorylated on Tyrosine 1054 (human).
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Primate
      • Bovine
      • Chicken
      • Horse
      • Zebrafish
      Species Reactivity NoteHuman tested. Expected to react with Mouse, Rat, primate, cow, chicken, horse, and Danio based on 100% sequnce homolgy.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KDR
      • VEGFR-2
      • VEGFR2
      • VEGFR
      • CD309
      • FLK1
      • EC 2.7.10.1
      Modifications
      • Phosphorylation
      Purification MethodDouble affinity purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35968 # Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 1356 amino acids; 151527 Da
      SUBUNIT: Interacts with SHB upon VEGF activation. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P35968 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain. ...
      Molecular Weight152 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      This lot detected VEGFR2 phosphorylated on Tyr1054 at a 1:1,000 dilution in PAE cells transfected with CSF/KDR and treated with 40ng/mL CSF-1 for 15 min. Cell lysate resolved via SDS-PAGE and transferred to PVDF (Immobilon-P).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-722 04053252403668

      Documentation

      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) - 2435684 2435684
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) - 2462815 2462815
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) - 2289168 2289168
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) - 2367284 2367284
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) - 3326211 3326211
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) - 3736714 3736714
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) - JBC1771113 JBC1771113
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) - NG1530265 NG1530265
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) -2712420 2712420
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) Polyclonal Antibody 2949186
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies