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07-1802 Anti-TAF3 Antibody

Detect TAF3 using this Anti-TAF3 Antibody validated for use in Chromatin Immunoprecipitation (ChIP) and Immunoprecipitation.

Anti-TAF3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: TAF3 UniProt Number: Q5VWG9
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07-1802
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, Rhesus Macaque, GpIP, ChIPRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-1802
      DescriptionAnti-TAF3 Antibody
      Alternate Names
      • Transcription initiation factor TFIID subunit 3
      • 140 kDa TATA box-binding protein-associated factor
      • TBP-associated factor 3
        Transcription initiation factor TFIID 140 kDa subunit
      • TAF(II)140
      • TAF140
      • TAF140
      • TAFII140
      Background InformationTAF3 is a subunit of TFIID that was initially identified through its interaction with a developmentally regulated transcription factor Bric a brac 1 (bab1), which is implicated in pattern formation and tissue morphogenesis in Drosophila. TFIID is a general or basal transcription factor that regulates initiation by allowing RNA polymerase II to recognize a core promotor. TAF3-TBPL2 complexes are found in myoblasts; however, upon differentiation into myotubes, they are replaced by TRF3-TAF3 complexes. Further research is still required to elucidate the roles and the development of the former to the latter complex.
      References
      Product Information
      FormatSerum
      Control
      • C2C12 cell lysate.
      PresentationRabbit polyclonal serum containing with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect TAF3 using this Anti-TAF3 Antibody validated for use in Chromatin Immunoprecipitation (ChIP) and Immunoprecipitation.
      Key Applications
      • Immunoprecipitation
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation: A representative lot immunoprecipitated TAF3 using chromatin from transfected cells grown under conditions that favor expression from the MyoD:EGFP transgene promoter over expression from the endogenous MyoD promoter.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human TAF3.
      HostRabbit
      Species Reactivity
      • Mouse
      • Rhesus Macaque
      • Guinea Pig
      Species Reactivity NoteDemonstrated to react with Mouse. Predicted to react with Human, Rhesus Macaque and Guinea Pig based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe highly conserved RNA polymerase II transcription factor TFIID (see TAF1; MIM 313650) comprises the TATA box-binding protein (TBP; MIM 600075) and a set of TBP-associated factors (TAFs), including TAF3. TAFs contribute to promoter recognition and selectivity and act as antiapoptotic factors.
      Gene Symbol
      • TAF3
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.

      SUBUNIT STRUCTURE: Belongs to the TFIID complex which is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Interacts with TAF10 via the histone fold. Interacts with TAF13, TBP, SAP130 and GCN5L2. Interacts with TBPL2.

      SUBCELLULAR LOCATION: Nucleus.
      Molecular Weight~180 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunoprecipitation in C2C12 cell lysate.

      Immunoprecipitation Analysis: A 1:1,000 dilution of this antibody detected TAF3 in 500 µg of C2C12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-1802 04053252757990