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07-1385 Anti-Lin28 Antibody

Anti-Lin28 Antibody detects level of Lin28 & has been published & validated for use in WB & IP.

Anti-Lin28 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Signaling Neuroscience, RNA Metabolism & Binding Proteins, RNA Binding Protein (RBP) Gene Symbol: LIN-28 , LIN28A UniProt Number: Q9H9Z2
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07-1385
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IPRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-1385
      DescriptionAnti-Lin28 Antibody
      Alternate Names
      • RNA-binding protein LIN-28
      Background InformationLin28 is a developmentally regulated RNA binding protein (RBP). Its down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.
      References
      Product Information
      FormatSerum
      PresentationRabbit Serum
      Quality LevelMQ100
      Applications
      ApplicationAnti-Lin28 Antibody detects level of Lin28 & has been published & validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation:
      Independent lab validated this antibody in immunoprecipitation. The antibody immunoprecipitated Lin28 from Tera-2 embryonic carcinoma and mouse ES cells using 2μL of antibody per reaction.
      Biological Information
      ImmunogenFull-length His-tagged Lin28 recombinant protein (human).
      HostRabbit
      SpecificityDetects Lin28
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman and Mouse. Other species have not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LIN-28
      • LIN28A
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression By similarity.

      SUBUNIT STRUCTURE: During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent By similarity.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus › nucleolus. Note= Nucleolar localization observed in 10 - 15% of the nuclei in differentiated myotubes By similarity.

      SPECIFICITY: Expressed in embryonic stem cells (ES cells), placenta and testis. Ref.7 Ref.8 Ref.9

      DEVELOPMENTAL STAGE: Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid. Ref.7 Ref.8 Ref.9

      INDUCTION: Can be negatively regulated by the interaction of microRNAs miR-125a and miR-125b with at least two miRNA responsive elements (miREs) in the 3'-UTR of this gene. These interactions may reduce both translation efficiency and mRNA abundance. Negatively regulated by retinoic acid. Ref.6 Ref.10

      DOMAIN: The CSD domain is required for function in muscle differentiation By similarity.

      SIMILARITY: Belongs to the lin-28 family.

      Contains 2 CCHC-type zinc fingers.

      Contains 1 CSD (cold-shock) domain.
      Molecular Weight25 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      This lot detected Lin28 at 1:1,000 dilution in Tera-2 embryonic carcinoma cell lysate resolved via SDS-PAGE and transferred to PVDF.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Store at -80°C for longer term storage. Avoid repeated freeze/thaws.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-1385 04053252281648

      Documentation

      Anti-Lin28 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Lin28 Antibody Certificates of Analysis

      TitleLot Number
      Anti-Lin28 3082653
      Anti-Lin28 - 2443073 2443073
      Anti-Lin28 - 2459568 2459568
      Anti-Lin28 - 2035082 2035082
      Anti-Lin28 - 4168644 4168644
      Anti-Lin28 - NG1579727 NG1579727
      Anti-Lin28 - NG1865042 NG1865042
      Anti-Lin28 Polyclonal Antibody 2930117

      References

      Reference overviewPub Med ID
      Selective blockade of microRNA processing by Lin28.
      Viswanathan, Srinivas R, et al.
      Science, 320: 97-100 (2008)  2008

      		18292307 18292307
      Depletion of human micro-RNA miR-125b reveals that it is critical for the proliferation of differentiated cells but not for the down-regulation of putative targets during differentiation.
      Lee, Yong Sun, et al.
      J. Biol. Chem., 280: 16635-41 (2005)  2005

      		15722555 15722555
      Micro-RNA regulation of the mammalian lin-28 gene during neuronal differentiation of embryonal carcinoma cells.
      Wu, Ligang and Belasco, Joel G
      Mol. Cell. Biol., 25: 9198-208 (2005)  2005

      		16227573 16227573

      Brochure

      Title
      RNA-Binding Protein Immunoprecipitation