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04-047 Anti-CUGBP2 Antibody, clone 1H2

This Anti-CUGBP2 Antibody, clone 1H2 is validated for use in WB for the detection of CUGBP2.

Anti-CUGBP2 Antibody, clone 1H2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Protein Trafficking Research Sub-Category: RNA Metabolism & Binding Proteins, RNA Binding Protein (RBP) Gene Symbol: CUGBP2, CUG-BP2, BRUNOL3, CELF2, CELF-2, Etr-3, ETR-3, ETR3, NAPOR-2, NAPOR2, NAPOR, hNAPOR UniProt Number: O95319
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04-047
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-047
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-CUGBP2 Antibody, clone 1H2
      References
      Product Information
      FormatPurified
      Presentation100 μg of Protein A purified mouse monoclonal IgG1 in 100μl of 1X PBS, pH 7.0, 0.1% Azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-CUGBP2 Antibody, clone 1H2 is validated for use in WB for the detection of CUGBP2.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenA synthetic peptide corresponding to amino acids 209-415 of human CuGBP2.
      Cloneclone 1H2
      HostMouse
      SpecificityCuGBP2
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMembers of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms.
      Gene Symbol
      • CUGBP2
      • CUG-BP2
      • BRUNOL3
      • CELF2
      • CELF-2
      • Etr-3
      • ETR-3
      • ETR3
      • NAPOR-2
      • NAPOR2
      • NAPOR
      • hNAPOR
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O95319 # RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre- mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury (By similarity). Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA (By similarity). Binds to an intronic RNA element responsible for the silencing of exon 21 splicing (By similarity). Binds to (CUG)n repeats (By similarity).


      SIZE: 508 amino acids; 54285 Da

      SUBUNIT: Found in a complex with APOBEC1 and ACF.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Accumulates in the cytoplasm after ionizing radiation (By similarity). Colocalizes with APOBEC1 and ACF. RNA-binding activity is detected in both nuclear and cytoplasmic compartments.

      TISSUE SPECIFICITY: Expressed in frontal cortex. Isoform 1 is expressed in brain and lung. Isoform 2 is expressed in heart, brain, placenta, lung, liver, kidney, skeletal muscle and pancreas. Isoform 4 is expressed in heart, lung, skeletal muscle, kidney and pancreas.
      DEVELOPMENTAL STAGE: Isoform 1 is expressed in fetal brain. Isoform 2 is expressed in fetal heart, brain, thymus, lung, liver, skeletal muscle, kidney and spleen. Isoform 4 is expressed in fetal heart, brain, thymus, lung and skeletal muscle.




      SIMILARITY: SwissProt: O95319 ## Belongs to the CELF/BRUNOL family. & Contains 3 RRM (RNA recognition motif) domains.
      Molecular Weight56 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      04-047 04053252581625

      Documentation

      Required Licenses

      Title
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      Anti-CUGBP2 Antibody, clone 1H2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CUGBP2 Antibody, clone 1H2 Certificates of Analysis

      TitleLot Number
      Anti-CuGBP2, clone 1H2 (Mouse monoclonal IgG1) - 2433335 2433335
      Anti-CuGBP2, clone 1H2 (Mouse monoclonal IgG1) 3066213
      Anti-CuGBP2, clone 1H2 (Mouse monoclonal IgG1) - 2074888 2074888
      Anti-CuGBP2, clone 1H2 - 3783547 3783547
      Anti-CuGBP2, clone 1H2 - DAM1460230 DAM1460230
      Anti-CuGBP2, clone 1H2 - DAM1817683 DAM1817683
      Anti-CuGBP2, clone 1H2 - JBC1913123 JBC1913123
      Anti-CuGBP2, clone 1H2 -2849462 2849462
      Anti-CuGBP2, clone 1H2_2830872 2830872

      References

      Reference overviewPub Med ID
      ETR-3 and CELF4 protein domains required for RNA binding and splicing activity in vivo
      Singh, Gopal, et al
      Nucleic Acids Res, 32:1232-41 (2004)  2004

      		14973222 14973222
      Dynamic antagonism between RNA-binding protein CUGBP2 and cyclooxygenase-2-mediated prostaglandin E2 in radiation damage
      Murmu, Nabendu, et al
      Proc Natl Acad Sci USA, 101:13873-8 (2004)  2004

      		15358864 15358864
      CUGBP2 plays a critical role in apoptosis of breast cancer cells in response to genotoxic injury
      Mukhopadhyay, Debnath, et al
      Ann N Y Acad Sci, 1010:504-9 (2003)  2003

      		15033780 15033780
      Expression and mutation analysis of BRUNOL3, a candidate gene for heart and thymus developmental defects associated with partial monosomy 10p
      Lichtner, P, et al
      J Mol Med, 80:431-42 (2002)  2002

      		12110949 12110949

      Brochure

      Title
      RNA-Binding Protein Immunoprecipitation

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies