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MABT530 Anti-N-cadherin Antibody, clone 6A9.2

This mouse monoclonal Anti-N-cadherin antibody, clone 6A9.2, Cat. No. MABT530 is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of N-cadherin.

Anti-N-cadherin Antibody, clone 6A9.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: CDH2, CDHN, NCAD UniProt Number: P19022
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MABT530
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT530
      Replaces04-1126
      DescriptionAnti-N-cadherin Antibody, clone 6A9.2
      Alternate Names
      • Cadherin-2
      • CD325
      • CDw325
      • N-cadherin
      • Neural cadherin
      Background InformationCadherin-2 (UniProt P19022; also known as CD325, CDw325, N-cadherin, Neural cadherin) is encoded by the CDH2 (also known as CDHN, NCAD) gene (Gene ID 1000) in human. N-Cadherin is a single-pass type I membrane protein that plays a role in calcium-dependent cell adhesion. N-cadherins preferentially interacts with themselves in a homophilic manner in connecting cells; may thus contribute to the sorting of heterogeneous cell types. Cadherins are synthesized as precursor proteins that are proteolytically cleaved to generate functional, mature proteins. Newly synthesized proN-cadherin (a.a. 1-906) is phosphorylated and proteolytically processed prior to transport to the plasma membrane. Cadherins are composed of an extracellular domain (a.a. 160-724 of human N-cadherin) with five homologous repeats that mediates adhesion, a single pass transmembrane domain (a.a. 725-745 of human N-cadherin), and a conserved cytoplasmic domain (a.a. 746-906 of human N-cadherin) that interacts with catenins to link cadherins to the actin cytoskeleton. N-cadherin is well expressed in endothelial cells and plays an essential role in the maturation and stabilization of normal vessels. However, it also promotes tumor cell survival, migration and invasion, and a high level of its expression is often associated with poor prognosis.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-N-cadherin antibody, clone 6A9.2, Cat. No. MABT530 is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of N-cadherin.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected N-cadherin in human cervical cancer, adrenal gland, and liver tissue sections.
      Biological Information
      ImmunogenGST/His-tagged recombinant human N-cadherin extracellular cadherin 1 domain fragment.
      Clone6A9.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6A9.2 targets an epitope within N-cadherin extracellular cadherin 1 domain.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDH2
      • CDHN
      • NCAD
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~140 kDa observed. Target band size appears larger than the calculated molecular weights of 99.81/97.23/82.03 kDa (prepro-/pro-/mature human N-cadherin) and 99.69/97.24/81.91 kDa (prepro-/pro-/mature rat N-cadherin) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in C6 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected N-cadherin in 10 µg of C6 rat glioma cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT530 04054839112324