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MABS810 Anti-MAP2K6, clone 3H12C8 Antibody

Anti-MAP2K6, clone 3H12C8 Antibody is an antibody against MAP2K6 for use in Western Blotting, Immunocytochemistry, Flow Cytometry, ELISA.

Anti-MAP2K6, clone 3H12C8 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: MAP2K6, MEK6, MKK6, PRKMK6, SKK3 UniProt Number: P52564
MABS810
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, FC, ELISAMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABS810
      Replaces04-383
      DescriptionAnti-MAP2K6, clone 3H12C8 Antibody
      Alternate Names
      • Dual specificity mitogen-activated protein kinase kinase 6
      • MAP kinase kinase 6
      • MAPKK 6
      • MAP2K6
      Background InformationDual specificity mitogen-activated protein kinase kinase 6 (MP2K6) is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MP2K6 phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
      References
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgG1ascitic fluid containing 0.03% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationAnti-MAP2K6, clone 3H12C8 Antibody is an antibody against MAP2K6 for use in Western Blotting, Immunocytochemistry, Flow Cytometry, ELISA.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Flow Cytometry
      • ELISA
      Application NotesImmunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected MAP2K6 in HeLa cells.

      Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected MAP2K6 in HeLa cells.

      ELISA: A representative lot was used on control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
      Biological Information
      ImmunogenPurified recombinant fragment of human MAP2K6 expressed in E. Coli.
      Clone3H12C8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • MAP2K6
      • MEK6
      • MKK6
      • PRKMK6
      • SKK3
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~38 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2, MCF-7 and NIH/3T3 cell lysates.

      Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected MAP2K6 in HepG2, MCF-7 and NIH/3T3 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS810 04055977172218

      Documentation

      Anti-MAP2K6, clone 3H12C8 Antibody Certificates of Analysis

      TitleLot Number
      Anti-MAP2K6, clone 3H12C8 -QVP1502050 QVP1502050
      Anti-MAP2K6, clone 3H12C8 -VP1609042 VP1609042
      Anti-MAP2K6, clone 3H12C8 Monoclonal Antibody VP1801124
      Anti-MAP2K6, clone 3H12C8 Monoclonal Antibody VP1704270

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.