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MABS434 Anti-PCSK9 Antibody, clone 9B3.1

Detect PCSK9 using this mouse monoclonal Anti-PCSK9, clone 9B3.1 Antibody, Cat. No. MABS434, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-PCSK9 Antibody, clone 9B3.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: PCSK9, HCHOLA3, LDLCQ1, NARC1, PSEC0052 UniProt Number: Q8NBP7
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MABS434
200 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), WBMCulture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMABS434
      DescriptionAnti-PCSK9 Antibody, clone 9B3.1
      Alternate Names
      • Proprotein convertase subtilisin/kexin type 9
      • NARC-1
      • Neural apoptosis-regulated convertase 1
      • PC9
      • Proprotein convertase 9
      • Subtilisin/kexin-like protease PC9
      Background InformationProprotein convertase subtilisin/kexin type 9 (UniProt Q8NBP7; also known as NARC-1, Neural apoptosis-regulated convertase 1, PC9, Proprotein convertase 9, Subtilisin/kexin-like protease PC9) is encoded by the PCSK9 (also known as HCHOLA3, LDLCQ1, NARC1, PSEC0052) gene (Gene ID 255738) in human. PCSK9 is a proprotein convertase expressed in the hepatocyte and to a lesser extent also in the intestine and kidney. PCSK9 is produced as a precusor protein with a signal peptide (a.a.1-30) and a propeptide sequence (a.a. 31-152) that undergo posttranslational autocleavage to yield the mature PCSK9 protein (a.a. 153-692). PCSK9 utilizes its proteolytic activity only in autocleavage during protein maturation, a process that is necessary for its secretion. Once secreted into the plasma, PCSK9 binds LDL receptor (LDL-R), causing LDL-R endocytosis and lysosomal degradation, effectively reducing cell surface LDL-R population. Both PCSK9 and LDL-R are transcribed by sterol-responsive element-binding protein 2 (SREBP2) in response to lowered cellular cholesterol level, allowing enhanced, but not unfettered cellular cholesterol uptake by LDL-R. PCSK9 also binds very low density lipoprotein (VLDL) receptor and apolipoprotein E receptor 2, and thereby interferes with uptake of circulating VLDL particles and apolipoproteins.
      References
      Product Information
      FormatCulture Supernatant
      PresentationMouse IgG2a hybridoma culture supernatant without azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect PCSK9 using this mouse monoclonal Anti-PCSK9, clone 9B3.1 Antibody, Cat. No. MABS434, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected NARC-1 (PCSK9) in human colon cancer, normal kidney and prostate tissue sections.

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected NARC-1 (PCSK9) in 10 µg of human colon tumor tissue lysate.
      Biological Information
      ImmunogenGST-tagged recombinant human mature NARC-1 (PCSK9) N-terminal fragment.
      Clone9B3.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 9B3.1 detects the precursor and mature forms of human PCSK9 (NARC-1) by targeting an epitope within the N-terminal region of the mature sequence.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PCSK9
      • HCHOLA3
      • LDLCQ1
      • NARC1
      • PSEC0052
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~75/60 kDa observed. 74.29 kDa (prepro-form; a.a. 1-692), 71.05 kDa (pro-form; a.a. 31-692), and 57.29 kDa (mature form; a.a. 153-692) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SW480 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this hybridoma culture supernatant detected NARC-1 (PCSK9) in 10 µg of SW480 human colon adenocarcinoma cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS434 04054839086168