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MABS1988 Anti-SREBP-2 Antibody, clone 22D5

Anti-SREBP-2, clone 22D5, Cat. No. MAB1988, a rabbit monoclonal antibody detects murine SREBP-2 by Western Blotting.

Anti-SREBP-2 Antibody, clone 22D5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Srebf2, Srebp2 UniProt Number: Q3U1N2
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MABS1988
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1988
      DescriptionAnti-SREBP-2 Antibody, clone 22D5
      Alternate Names
      • Sterol regulatory element-binding protein 2
      • SREBP2
      • Sterol regulatory element-binding transcription factor 2
      Background InformationSterol regulatory element-binding protein 2 (UniProt: Q3U1N2; also known as SREBP-2, Sterol regulatory element-binding transcription factor 2) is encoded by the Srebf2 (also known as Srebp2) gene (Gene ID: 20788) in murine species. SREBPs are a family of transcription factors that regulate lipid homeostasis by controlling the expression of a range of enzymes that are required for endogenous cholesterol, fatty acid, triacylglycerol, and phospholipid synthesis. The three SREBP isoforms known as, SREBP-1a, SREBP-1c, and SREBP-2, have different roles in lipid synthesis. SREBP-1 and SREBP-2 proteins share 47% of homology. SREBP-2 is mainly involved in cholesterol synthesis and SREBP-1c is mainly involved in fatty acid synthesis and insulin induced glucose metabolism and SREBP-1a isoform is involved in both of these pathways. SREBPs are synthetized as inactive precursor proteins that are bound to the endoplasmic reticulum membranes and upon activation, the precursor is cleaved off in a two-step process to release the N-terminal active domain in the nucleus. SREBP precursors are organized into three domains - an N-terminal domain that contains the transactivation domain, a region rich in serine and proline, and the bHLH-LZ region for DNA binding and dimerization. Sterols are shown to inhibit the cleavage of the precursor protein and the mature nuclear form is rapidly catabolized, thereby reducing transcription. It regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway. It binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes. The hepatic overexpression of SREBP-2 isoform in mice causes a preferential induction of genes involved in cholesterol biosynthesis. (Ref.: Eberle, D et al. (2004) Biochimie 86(11); 839-48).
      References
      Product Information
      FormatUnpurified
      PresentationRabbit monoclonal antibody in cell culture supernatant without azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SREBP-2, clone 22D5, Cat. No. MAB1988, a rabbit monoclonal antibody detects murine SREBP-2 by Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected SREBP-2 in 10 µg of HEK293 and HeLa cell lysates.

      Western Blotting Analysis: A representative lot detected SREBP-2 in Western Blotting applications (McFarlane, M.R., et. al. (2015). J Lipid Res. 56(8):1560-71).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 219 amino acids from the N-terminal region of murine Sterol regulatory element-binding protein 2.
      Clone22D5
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityClone 22D5 specifically detected Sterol regulatory element-binding protein 2. It targets an epitope within the 219 amino acids from the N-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Srebf2
      • Srebp2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~122 kDa observed; 122.91 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SREBP-2 in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1988 04054839193651

      Documentation

      Anti-SREBP-2 Antibody, clone 22D5 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-SREBP-2 Antibody, clone 22D5 Certificates of Analysis

      TitleLot Number
      Anti-SREBP-2, clone 22D5 - 3272232 3272232
      Anti-SREBP-2, clone 22D5 - 3647665 3647665
      Anti-SREBP-2, clone 22D5 - 3975475 3975475
      Anti-SREBP-2, clone 22D5 - 4042560 4042560
      Anti-SREBP-2, clone 22D5 - 4070057 4070057
      Anti-SREBP-2, clone 22D5 - 4120677 4120677
      Anti-SREBP-2, clone 22D5 - 4228985 4228985
      Anti-SREBP-2, clone 22D5 Monoclonal Antibody 2970988
      Anti-SREBP-2, clone 22D5 Monoclonal Antibody 2966303
      Anti-SREBP-2, clone 22D5 Monoclonal Antibody 3000905
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