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MABN1596 Anti-Pan Shc Antibody, clone 3A12.1

Anti-Pan Shc Antibody, clone 3A12.1 is an antibody against Pan Shc for use in Western Blotting, Immunohistochemistry.

Anti-Pan Shc Antibody, clone 3A12.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Signaling Neuroscience Gene Symbol: SHC1, SHC, SHCA UniProt Number: P29353
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MABN1596
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1596
      DescriptionAnti-Pan Shc Antibody, clone 3A12.1
      Alternate Names
      • p46Shc
      • p52Shc
      • p66Shc
      • SHC-transforming protein 1
      • SH2 domain protein C1
      • SHC-transforming protein 3
      • SHC-transforming protein A
      • Src homology 2 domain-containing-transforming protein C1
      Background InformationSHC-transforming protein 1 (UniProt P29353; also known as SH2 domain protein C1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1) is encoded by the SHC1 (also known as SHC, SHCA) gene (Gene ID 6464) in human. Originally identified as an SH2-containing proto-oncogene involved in growth factor signaling, Shc proteins function as adaptor molecules recruited by numerous receptors (e.g. integrins, GPCRs, and receptors for growth factors, antigens, cytokines, and hormones) for signling. In addition to the ubiquitously expressed Shc (a.k.a. ShcA by SHC1/SHCA gene), there exist also ShcB (by SHC2/SHCB gene) and ShcC (by SHC3/SHCC gene), which are predominantly expressed in neuronal cells. Alternative splicings result in six ShcA isoforms, including p66Shc, p52Shc, and p46Shc. All ShcA isoforms possess two distinct domains, PTB (a.a. 156-339) and SH2 (a.a. 488-579), that bind phosphotyrosine-containing sequences, as well as a central region (CH1; a.a. 340-487) that contains critical tyrosine phosphorylation sites. The phosphotyrosine-binding (PTB) domain is also referred to as phosphotyrosine-interaction domain or PID.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Pan Shc Antibody, clone 3A12.1 is an antibody against Pan Shc for use in Western Blotting, Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Shc proteins in human prostate cancer, as well as in normal breast and tonsil tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human Shc C-terminal region fragment.
      EpitopeC-terminal region.
      Clone3A12.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3A12.1 was raised against a C-terminal region sequence present in all six human SHC1 spliced isoforms reported by UniProt (P29353).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SHC1
      • SHC
      • SHCA
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~47-63 kDa observed. 62.82 kDa (isoform p66Shc), 51.61 kDa (isoform p52Shc), 46.67 kDa (isoform p46Shc), 40.42 kDa (isoform 5), 62.89 kDa (isoform 6), 51.68 kDa (isoform 7) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SH-SY5Y cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Shc proteins in 10 µg of SH-SY5Y cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1596 04055977350944