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MABC990 Anti-TIM4/TIMD-4 Antibody, clone Mat4-9-5

Anti-TIM4/TIMD-4 Antibody, clone Mat4-9-5 is an antibody against TIM4 for use in Flow Cytometry.

Anti-TIM4/TIMD-4 Antibody, clone Mat4-9-5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: Timd4, Tim4 UniProt Number: Q6U7R4
MABC990
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFCAHmPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC990
      DescriptionAnti-TIM4/TIMD-4 Antibody, clone Mat4-9-5
      Alternate Names
      • T-cell immunoglobulin and mucin domain-containing protein 4
      • TIMD-4
      • Spleen, mucin-containing, knockout of lymphotoxin protein
      • SMUCKLER
      • T-cell immunoglobulin mucin receptor 4
      • TIM-4
      • T-cell membrane protein 4
      Background InformationT-cell immunoglobulin and mucin domain-containing protein 4 (UniProt Q6U7R4; also known as SMUCKLER, Spleen, mucin-containing, knockout of lymphotoxin protein, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4, TIM-4, TIMD-4) is encoded by the Timd4 (also known as Tim4) gene (Gene ID 276891) in murine species. TIM-4 is initially produced with a signal peptide (a.a. 1-22), the removal of which yields the mature type I membrane protein with a large extracellular region (a.a. 23-279), a transmembrane segment (a.a. 280-300), and a small cytoplasmic tail (a.a. 301-343). TIM-4 is expressed by subsets of macrophages, including resident peritoneal macrophages and splenic MOMA-1+-marginal zone macrophages and is shown to bind phosphatidylserine in vitro and support the engulfment of apoptotic cells when expressed in mouse NIH3T3 cells. Milk Fat Globule EGF Factor VIII (MFG-E8) is a soluble protein that also binds phosphatidylserine on apoptotic cells and acts as a bridge between dead cells and phagocytes. MFG-E8 is secreted from a different subset of macrophages and immature dendritic cells than those which express TIM-4. MFG-E8 and Tim4 double knockout mice suffer from autoimmune diseases due to the production of high level of autoantibodies against the large number of unengulfed apoptotic cells.
      Product Information
      FormatPurified
      PresentationPurified Armenian hamster monoclonal IgG antibody in PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-TIM4/TIMD-4 Antibody, clone Mat4-9-5 is an antibody against TIM4 for use in Flow Cytometry.
      Key Applications
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: A representative lot, when conjugated with biotin, was used for flow cytometry analysis and detected Tim4 expression on the surface of CD11b-positive peritoneal cells from wild-type, but not Tim4-knockout mice (Miyanishi, M., et al. (2012). Int. Immunol. 24(9):551-559).
      Biological Information
      ImmunogenHuman IgG Fc-tagged recombinant protein corresponding to the extracellular domain of mouse TIM4/TIMD-4.
      EpitopeExtracellular domain.
      CloneMat4-9-5
      ConcentrationPlease refer to lot specific datasheet.
      HostArmenian Hamster
      IsotypeIgG
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Timd4
      • Tim4
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight41 kDa calculated.
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in murine Tim4-overexpressing Ba/F3 cells.

      Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected Tim4 surface expression on murine Tim4-overexpressing Ba/F3 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABC990 04055977314236