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MABC32-AF488 Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 Antibody, Alexa Fluor® 488 Conjugate

Detect p62 using this mouse monoclonal Anti-p62 (Sequestosome-1) antibody, clone 11C9.2, Alexa Fluor® 488 Conjugate, Cat. No. MABC32-AF488, validated for use in Flow Cytometry and Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: SQSTM1, ORCA, OSIL UniProt Number: Q13501
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MABC32-AF488
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, MFC, ICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue NumberMABC32-AF488
      DescriptionAnti-p62 (Sequestosome-1) Antibody, clone 11C9.2 Antibody, Alexa Fluor® 488 Conjugate
      Alternate Names
      • EBI3-associated protein of 60 kDa
      • EBIAP
      • p60
      • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
      • Ubiquitin-binding protein p62
      Background InformationSequestosome-1 (UniProt: Q13501; also known as EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62) is encoded by the SQSTM1 (also known as ORCA, OSIL) gene (Gene ID 8878) in human. Sequestosome-1 is a multifunctional protein that binds ubiquitin and regulates the activation of the nuclear factor kappa-B (NF-kappaB) signaling pathway. It functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 (TRAF6) to mediate the activation of NF-kappaB in response to upstream signals. Defects in sequestosome-1 are a cause of Paget disease of bone (PDB), a metabolic bone disease affecting the axial skeleton and is characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Mutations in SQSTM1 gene can lead to frontotemporal dementia and/or amyotrophic lateral sclerosis 3. Frontotemporal dementia is characterized by frontal and temporal lobe atrophy associated with neuronal loss, gliosis, and dementia. Amyotrophic lateral sclerosis is characterized by the death of motor neurons in the brain, brainstem, and spinal cord, resulting in fatal paralysis.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified mouse monoclonal antibody IgM in PBS with 1.5% BSA and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect p62 using this mouse monoclonal Anti-p62 (Sequestosome-1) antibody, clone 11C9.2, Alexa Fluor® 488 Conjugate, Cat. No. MABC32-AF488, validated for use in Flow Cytometry and Immunocytochemistry.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p62 Sequestosome-1 in A431 and NIH/3T3 cells.

      Flow Cytometry Analysis: A 1:400 dilution from a representative lot detected p62 Sequestosome-1 in one million HeLa cells.
      Biological Information
      ImmunogenGST-tagged recombinant fragment of 200 amino acids from the internal region of human p62 (Sequestosome-1).
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 11C9.2, Alexa Fluor® 488 Conjugate detects p62 in HeLa cells.
      IsotypeIgM
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Species Reactivity NoteHuamn, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SQSTM1
      • ORCA
      • OSIL
      Purification MethodPurified by ion-exchange chromatography.
      UniProt Number
      Molecular Weight47.7 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected p62 (Sequestosome-1) in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC32-AF488 04054839139147

      Documentation

      Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 Antibody, Alexa Fluor® 488 Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 Antibody, Alexa Fluor® 488 Conjugate Certificates of Analysis

      TitleLot Number
      Anti-p62 (Sequestosome-1), clone -Q2800239 Q2800239
      Anti-p62 (Sequestosome-1), clone 11C9.2, Alexa Fluor® 488 Conjugate - 3172399 3172399
      Anti-p62 (Sequestosome-1), clone 11C9.2, Alexa Fluor® 488 Conjugate - 3310104 3310104
      Anti-p62 (Sequestosome-1), clone 11C9.2, Alexa Fluor® 488 Conjugate - 3481425 3481425
      Anti-p62 (Sequestosome-1), clone 11C9.2, Alexa Fluor® 488 Conjugate - 3995667 3995667
      Anti-p62 (Sequestosome-1), clone 11C9.2, Alexa Fluor® 488 Conjugate - 4202323 4202323
      Anti-p62 (Sequestosome-1), clone 11C9.2, Alexa Fluor® 488 Conjugate Monoclonal Antibody 3040404