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MABC1158 Anti-phospho-MLKL (Ser345) Antibody, clone 7C6.1

Detect phospho MLKL Ser345 using this mouse monoclonal Anti-phospho-MLKL (Ser345), clone 7C6.1, Cat. No. MABC1158. It has been tested in ELISA, Immunocytochemistry, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: Mlkl UniProt Number: Q9D2Y4
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MABC1158
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MICC, WB, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1158
      DescriptionAnti-phospho-MLKL (Ser345) Antibody, clone 7C6.1
      Alternate Names
      • Mixed lineage kinase domain-like protein
      Background InformationMixed lineage kinase domain-like protein (UniProt: Q9D2Y4; also known as MLKL) is encoded by the Mlkl gene (Gene ID: 74568) in murine species. MLKL is a pseudokinase that plays a key role in TNF-induced necroptosis. MLKL is highly expressed in thymus, colon, intestine, liver, spleen and lung and is expressed at much lower level in skeletal muscle, heart and kidney. It is not detected in brain. MLKL is activated following phosphorylation by RIPK3 that induces a conformational switch, which is essential for necroptosis. The protein kinase domain of MLKL is catalytically inactive, but contains a pseudoactive site with an interaction between Lys-219 and Gln-343 residues. Upon phosphorylation by RIPK3, MLKL undergoes an active conformation and homotrimerization through its amino-terminal coiled-coil domain. Phosphorylation is also essential for homotrimerization of MLKL and its subsequent localization to the plasma membrane. Plasma membrane localization of MLKL is considered to be essential for Ca2+ influx, which is an early event of TNF-induced necroptosis. (Ref.: Cai, Z et al. (2014). Nat. Cell Biol. 16(1): 55-65).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect phospho MLKL Ser345 using this mouse monoclonal Anti-phospho-MLKL (Ser345), clone 7C6.1, Cat. No. MABC1158. It has been tested in ELISA, Immunocytochemistry, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • ELISA
      Application NotesELISA Analysis: A representative lot detected phospho-MLKL (Ser345) in ELISA applications (Rodriguez, D.A., et. al. (2016). 23(1):76-88).

      Immunocytochemistry Analysis: A representative lot detected phospho-MLKL (Ser345) in Immunocytochemistry applications (Rodriguez, D.A., et. al. (2016). 23(1):76-88).

      Western Blotting Analysis: A representative lot detected phospho-MLKL (Ser345) in Western Blotting applications (Rodriguez, D.A., et. al. (2016). 23(1):76-88).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids surrounding Ser345 from the C-terminal half of murine Mixed lineage kinase domain-like protein (MLKL).
      Clone7C6.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 7C6.1 specifically detects MLKL phosphorylalated on Ser345. It targets a sequence of 19 amino acids surrounding Ser345 in the C-terminal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Mlkl
      Modifications
      • Phosphorylation
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~54 kDa observed; 54.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MLKL -/- mouse embryonic fibroblasts expressing doxycycline-inducible wild-type MLKL and treated with TNF alpha and Z-VAD-FMK.

      Western Blotting Analysis: A 1:4,000 dilution of this antibody detected phospho-MLKL Ser345 in 20 ug of lysate from MLKL -/- mouse embryonic fibroblasts expressing doxycycline-inducible wild-type MLKL-Flag-C construct and treated with TNF alpha (10 ng/mL) and Z-VAD-FMK (25 uM).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1158 04054839174469

      Documentation

      Anti-phospho-MLKL (Ser345) Antibody, clone 7C6.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-MLKL (Ser345) Antibody, clone 7C6.1 Certificates of Analysis

      TitleLot Number
      Anti-phospho-MLKL (Ser345), clone 7C6.1 - 3471964 3471964
      Anti-phospho-MLKL (Ser345), clone 7C6.1 - 3784171 3784171
      Anti-phospho-MLKL (Ser345), clone 7C6.1 - 4181453 4181453
      Anti-phospho-MLKL (Ser345), clone 7C6.1 - 4250381 4250381
      Anti-phospho-MLKL (Ser345), clone 7C6.1 MONOCLONAL ANTIBODY Q2821638
      Anti-phospho-MLKL (Ser345), clone 7C6.1 Monoclonal Antibody 3017555
      Anti-phospho-MLKL (Ser345), clone 7C6.1 Monoclonal Antibody 2990495
      Anti-phospho-MLKL (Ser345), clone 7C6.1 Monoclonal Antibody 2955658
      Anti-phospho-MLKL (Ser345), clone 7C6.1 Monoclonal Antibody 3083580
      Anti-phospho-MLKL (Ser345), clone 7C6.1 Monoclonal Antibody 3094607
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