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MABC1143 Anti-MED28 Antibody, clone 7E1

Detect MED28 using this mouse monoclonal Anti-MED28 Antibody, clone 7E1, Cat. No. MABC1143, validated for use in Electron Microscopy, Immunocytochemistry, and Western Blotting.

Anti-MED28 Antibody, clone 7E1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: MED28, EG1, FKSG20 UniProt Number: Q9H204
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MABC1143
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, MkICC, WB, EMMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1143
      DescriptionAnti-MED28 Antibody, clone 7E1
      Alternate Names
      • Mediator of RNA polymerase II transcription subunit 28
      • Endothelial-derived protein 1
      • Magicin
      • Mediator complex subunit 28
      • Merlin and Grb2-interacting cytoskeletal protein
      • Tumor angiogenesis marker EG-1
      Background InformationMediator of RNA polymerase II transcription subunit 28 (UniProt Q9H204; also known as Endothelial-derived protein 1, Magicin, Mediator complex subunit 28, Merlin and Grb2-interacting cytoskeletal protein, Tumor angiogenesis marker EG-1) is encoded by the MED28 (also known as EG1, FKSG20) gene (Gene ID 80306) in human. MED28 (Magicin) is a subunit of the Mediator complex that is composed of at least 30 subunits and plays an essential role in transcriptional regulation by bridging RNA polymerase II transcriptional machinery and DNA-bound regulatory factors. MED28 is reported to mediate cell surface receptor-mediated signaling and associate with Src-family kinases (e.g. Fyn, Src and Lck), as well as with the Neurofibromatosis 2 (NF2) tumor suppressor merlin. MED28 is associated with both the cytoskeleton and the nucleus, indicating a role as an adaptor/scaffolding protein to relay cellular signals to the cytoskeleton and the nucleus. Homozygous Med28-deletion causes peri-implantation lethality with a loss of pluripotency within the inner cell mass (ICM) of the blastocyst. In addition, Med28-/- embryonic fibroblasts (MEFs) lose the capability to reprogram and Med28 overexpression augments reprogramming efficiency. Upregulated MED28 expression is associated with poor outcome in breast cancer patients, and targeted MED28 downregulation decreases breast cancer cell proliferation and tumor growth.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect MED28 using this mouse monoclonal Anti-MED28 Antibody, clone 7E1, Cat. No. MABC1143, validated for use in Electron Microscopy, Immunocytochemistry, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Electron Microscopy
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected MED28 in 10 µg of human embryonic kidney HEK293 cell lysates.

      Electron Microscopy Analysis: A representative lot detected MED28 (Magicin) immunoreactivity on cortical actin filaments, colocalizing with that of merlin by immunogold staining of 1% glutaraldehyde-fixed, 0.1% SDS-treated CAD mouse neuronal cells (Wiederhold, T., et al. (2004). Oncogene. 23(54):8815-8825).

      Immunocytochemistry Analysis: A representative lot immunostained 4% paraformaldehyde-fixed, 0.25% Triton X-100-permeabilized wild-type, but not Med28-/-, mouse blastocysts, as well as wild-type MEF-dervied iPSC colonies (Li, L., et al. (2015). PLoS One. 10(10):e0140192).

      Immunocytochemistry Analysis: A representative lot detected MED28 (Magicin) immunoreactivity colocalized with that of phalloidin-stained actin filament by flourescent immunocytochemistry staining of 0.1% SDS-treated CAD mouse neuronal cells (Wiederhold, T., et al. (2004). Oncogene. 23(54):8815-8825).

      Western Blotting Analysis: A representative lot detected a higher MED28 expression in mouse embryonic stem cells (ESCs) than in embryonic fibroblasts (MEFs). Clone 7E1 detected Cre expression-induced MED28 knockdown in MEFs with floxed Med28 alleles (Li, L., et al. (2015). PLoS One. 10(10):e0140192).

      Western Blotting Analysis: A representative lot detected ~24 kDa MED28 (Magicin) in human (F293, Gli238, HEK293T, HeLa, MeT5A, T-47D), monkey (COS-7), and mouse (CAD) cell lysates, as well as in Merlin immunoprecipitates from T-47D cytosolic/heavy membrane fractions (Cyt/HMF) and whole cell lysate (Wiederhold, T., et al. (2004). Oncogene. 23(54):8815-8825).
      Biological Information
      ImmunogenGST-tagged recombinant human MED28 lacking N-terminal 12 amino acids.
      Clone7E1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 7E1 detected MED28 in wild-type, but not Med28-/- murine cells. Clone 7E1 detected Cre expression-induced MED28 knockdown in MEFs with floxed Med28 alleles (Li, L., et al. (2015). PLoS One. 10(10):e0140192).
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Monkey
      Species Reactivity NoteHuman, Monkey, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MED28
      • EG1
      • FKSG20
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~20 kDa observed. 19.52 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in RAW267.7 murine macrophage lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected MED28 in 10 µg of RAW267.7 murine macrophage lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1143 04054839090080