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MAB5456-C Anti-Gonadotropin-Releasing Hormone Antibody, NT, clone HU11B (Ascites Free)

Anti-Gonadotropin-Releasing Hormone Antibody, NT, clone HU11B (Ascites Free) is an antibody against Gonadotropin-Releasing Hormone for use in Immunohistochemistry (Paraffin), Affinity Binding Assay, Immunofluorescence, Electron Microscopy.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: Gnrh1, Gnrh, Lhrh1, Lnrh UniProt Number: P13562
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MAB5456-C
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Rhesus MacaqueIH(P), ABA, IF, EMMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB5456-C
      DescriptionAnti-Gonadotropin-Releasing Hormone Antibody, NT, clone HU11B (Ascites Free)
      Alternate Names
      • Gonadotropin-releasing hormone I
      • GnRH-I
      • Luliberin I
      • Luteinizing hormone-releasing hormone I
      • LH-RH I
      Background InformationGonadotropin-releasing hormone I (also known as GnRH, GnRH-I, Gonadoliberin-1, Gonadoliberin I, LHRH, LH-RH I, Luteinizing hormone-releasing hormone I) is encoded by the Gnrh1 (also known as Gnrh, Lhrh1, Lnrh) gene (Gene ID 14714) in murine species. GnRH/LHRH is initially produced as a 90-amino acid progonadoliberin-1 precursor protein (UniProt P13562), removal of the signal peptide (a.a. 1-21) yields progonadoliberin-1 (a.a. 22-90), which is further processed by a proteolytic cleavage to release the GnRH/LHRH decapeptide (a.a. 22-31) and the 56-a.a. prolactin release-inhibiting factor 1 (a.a. 35-90). The LHRH decapeptide functions as the primary endocrine link between the brain and the reproductive system. It plays a primary neuroendocrine role in orchestrating the developmental processes associated with sexual development in immature mammals and also in maintaining normal reproductive function in adults. LHRH-producing neurons are located mainly in the lateral anterior hypothalamus, septum, and medial preoptic area, from where they extend axons towards the median eminence and relese the hormone into the hypothalamo-hypophyseal portal blood vessels in an episodic or pulsatile manner.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Gonadotropin-Releasing Hormone Antibody, NT, clone HU11B (Ascites Free) is an antibody against Gonadotropin-Releasing Hormone for use in Immunohistochemistry (Paraffin), Affinity Binding Assay, Immunofluorescence, Electron Microscopy.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Affinity Binding Assay
      • Immunofluorescence
      • Electron Microscopy
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Gonadotropin-Releasing Hormone in human prostate cancer tissue.
      Affinity Binding Assay: A representative lot captured C-terminally blocked GnRH-I/LHRH (LHRH-NH2), but not LHRH with a free C-terminal end (LHRH-COOH). Clone HU11B exhibited reduced affinity toward N-terminally truncated LHRH-NH2 and failed to recognize pro-LHRH, Atrial Naturetic Factor/ANF, Growth hormone-releasing hormone/GHRH, Oxytocin, Somatostatin, Thyrotropin-releasing hormone/TRH, and Vasoactive intestinal peptide/VIP (Urbanski, H.F., et al. (1991). Biol. Reprod. 44(4):681-686).
      Immunofluorescence Analysis: A representative lot detected GnRH neurons in brain sections from female rhesus monkeys by fluorescent immunohistochemistry (Naugle, M.M., and Gore, A.C. (2014). Neuroendocrinology 100:334-346).
      Immunofluorescence Analysis: A representative lot detected pregnancy stage-dependent GnRH-I immunoreactivity in the luminal epithelium of rat oviduct by fluorescent immunohistochemistry using paraformaldehyde-fixed cryosections (Sengupta, A., et al. (2007). J. Histochem. Cytochem. 55(5):525-534).
      Immunofluorescence Analysis: A representative lot detected GnRH-I immunoreactivity in frozen mouse hypothalamic sections by fluorescent immunohistochemistry (Olcese, J., et al. (2003). Neuroreport. 14(4):613-618).
      Electron Microscopy: A representative lot detected GnRH-I immunoreactivity associated with the luminal epithelium secretory vesicles of rat oviduct by EM using paraformaldehyde-fixed LR wihite sections from rats at day 16 of pregnancy (Sengupta, A., et al. (2007). J. Histochem. Cytochem. 55(5):525-534).
      Immunohistochemistry Analysis: A representative lot detected GnRH-I immunoreactivity throughout the hypothalamus in paraformalin-fixed, paraffin-embedded South American plains vizcacha (Lagostomus maximus) brain sections (Dorfman, V.B., et al. (2011). J. Mol. Histol. 42(4):311-321).
      Immunohistochemistry Analysis: A representative lot detected GnRH-I-positie neurons in formalin-fixed, paraffin-embedded mouse brain sections (Bergman, J.E., et al. (2010). Eur. J. Hum. Genet. 18(2):171-177).
      Immunohistochemistry Analysis: A representative lot detected GnRH-I immunoreactivity in paraformaldehyde-fixed rat brain vibratome sections (Urbanski, H.F., et al. (1991). Biol. Reprod. 44(4):681-686).
      Biological Information
      ImmunogenMouse GnRH (QHWSYGLRPG) conjugated to bovine thyroglobulin.
      EpitopeN-terminus.
      CloneHU11B
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone HU11B reacts with C-terminally blocked GnRH-I/LHRH (LHRH-NH2), but not LHRH with a free C-terminal end (LHRH-COOH). Clone HU11B exhibits reduced affinity toward N-terminally truncated LHRH-NH2 and failed to recognize pro-LHRH, Atrial Naturetic Factor/ANF, Growth hormone-releasing hormone/GHRH, Oxytocin, Somatostatin, Thyrotropin-releasing hormone/TRH, and Vasoactive intestinal peptide/VIP (Urbanski, H.F., et al. (1991). Biol. Reprod. 44(4):681-686).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Rhesus Macaque
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Gnrh1
      • Gnrh
      • Lhrh1
      • Lnrh
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight10 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human prostate tissue.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Gonadotropin-Releasing Hormone in human prostate tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB5456-C 04055977312409

      Documentation

      Anti-Gonadotropin-Releasing Hormone Antibody, NT, clone HU11B (Ascites Free) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Gonadotropin-Releasing Hormone Antibody, NT, clone HU11B (Ascites Free) Certificates of Analysis

      TitleLot Number
      Anti-Gonadotropin-Releasing Hormone, NT, clone HU11B (Ascites Free) - 3789387 3789387
      Anti-Gonadotropin-Releasing Hormone, NT, clone HU11B (Ascites Free) - 3840350 3840350
      Anti-Gonadotropin-Releasing Hormone, NT, clone HU11B (Ascites Free) - 4076697 4076697
      Anti-Gonadotropin-Releasing Hormone, NT, clone HU11B (Ascites Free) -Q2638546 Q2638546

      Brochure

      Title
      Neuroscience Solutions for productive research