Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
Catalogue Number
Ordering Description
Qty/Pack
List
This item has been added to favorites.
Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
This item has been added to favorites.
Species
Panel Type
Selected Kit
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
96-Well Plate
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
This item has been added to favorites.
The Product Has Been Added To Your Cart
You can now customize another kit, choose a premixed kit, check out or close the ordering tool.
Cyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation on by various kinases, including PKA and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-CREB Antibody, clone LB9, Ascites Free is an antibody against CREB for use in Western Blotting, Immunohistochemistry (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Western Blotting Analysis: 1.0 µg/mL of this antibody from a representative lot detected CREB in 10 µg of NIH/3T3 and PC12 cell lysate. Immunohistochemistry Analysis: An 1:50-250 dilution of this antibody from a representative lot detected CREB in human liver, colon, and tonsil tissue sections. Western Blotting Analysis: Representative lots detected CREB in primary mouse astrocytes and mouse dorsal hippocampal tissue lysates (Orr, A.G., et al. (2015). Nat. Neurosci. 18(3):423-434; Yiu, A.P., et al. (2011). Neuropsychopharmacology. 36(11):2169-2186). Western Blotting Analysis: Representative lots detected CREB in primary human fibroblast and SH-SY5Y human neuroblastoma cell lysates (Maj, M., et al. (2011). Mitochondrion. 11(2):264-272; Fukuda, Y., et al. (2010). Mol. Cell Neurosci. 45(3):226-233). Immunohistochemistry Analysis: A representative lot immunostained total CREB in dorsal hippocampal tissue sections from paraformaldehyde-perfused mice (Yiu, A.P., et al. (2011). Neuropsychopharmacology. 36(11):2169-2186).
Biological Information
Immunogen
Recombinant CREB-A protein.
Clone
LB9
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone LB9 reactivity towarad spliced isoform CREB-B and htCREB (isoform 3) has not been determined.
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected CREB in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
