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MAB1924-C Anti-Laminin alpha5 Antibody, clone 4C7 (Ascites Free)

Anti-Laminin alpha5, clone 4C7 (Ascites Free), Cat. No. MAB1924-C, is a mouse monoclonal antibody that detects Laminin alpha 5 and has been tested for use in ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and neutralization.

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MAB1924-C
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB1924-C
      DescriptionAnti-Laminin alpha5 Antibody, clone 4C7 (Ascites Free)
      Alternate Names
      • Laminin-10 subunit alpha
      • Laminin-11 subunit alpha
      • Laminin-15 subunit alpha
      Background InformationLaminin subunit alpha-5 (UniProt: O15230; also known as Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha) is encoded by the LAMA5 (also known as KIAA0533, KIAA1907) gene (Gene ID: 3911) in human. Laminins are a family of heterotrimeric extracellular glycoproteins consisting of alpha, beta, and gamma chains. Laminins mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Thus far five alpha, three beta, and three gamma subunits are known, which assemble into at least 15 different laminin molecules. The alpha, beta, and gamma chains are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-5 is a subunit of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). It contains 22 laminin EGF-like domains, 5 5 laminin G-like domains, 1 laminin IV type A domain, and 1 laminin N-terminal domain. Laminin 5-alpha is expressed in heart, lung, kidney, muscle, pancreas, retina, and placenta. Very little or no expression is observed in brain and liver.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing PBS.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Laminin alpha5, clone 4C7 (Ascites Free), Cat. No. MAB1924-C, is a mouse monoclonal antibody that detects Laminin alpha 5 and has been tested for use in ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and neutralization.
      Key Applications
      • Neutralizing
      • Immunofluorescence
      • ELISA
      • Immunoprecipitation
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A representative lot detected Laminin alpha 5 in immunostained basement membranes in O.T.C.-embedded fetal and adult human lung cryosections (Pierce, R.A., et al. (2000). Am. J. Respir. Cell Mol. Biol. 23(6):742-747).

      Neutralizing Analysis: A representative lot detected Laminin alpha 5 in adhesion of sickle cell patients (Hb SS) red blood cells (RBCs) to human placental laminin-coated surface (Lee, S.P., et al. (1998). Blood. 92(8):2951-2958).

      ELISA Analysis: A representative lot detected Laminin alpha 5 in recombinant human (rh) Laminin-10 ( 5 1 1) and Laminin-11 ( 5 2 1), but not Laminin-8 ( 4 1 1) (Wondimu, Z., et al. (2013). PLoS One. 8(1):e53648).

      Immunofluorescence Analysis: A representative lot detected Laminin alpha 5 in immunoreactivity in human skin and kidney cryosections (Wondimu, Z., et al. (2013). PLoS One. 8(1):e53648).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Laminin alpha 5 in Laminin-10 ( 5 1 1) from A549 culture medium (Wondimu, Z., et al. (2013). PLoS One. 8(1):e53648).
      Biological Information
      ImmunogenFull length purified human laminin alpha 5.
      Clone4C7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4C7 reacts with the globular domain of the alpha5 chain of intact laminin and blocks the neurite stimulating activity of laminin.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LAMA5
      • KIAA0533
      • KIAA1907
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~260 kDa observed; 399.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunoprecipitation in A431 cell lysate.

      Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Laminin alpha 5 in 500 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1924-C 04054839253102

      Documentation

      Anti-Laminin alpha5 Antibody, clone 4C7 (Ascites Free) Certificates of Analysis

      TitleLot Number
      Anti-Laminin alpha5, clone 4C7 (Ascites Free) - 3552717 3552717
      Anti-Laminin alpha5, clone 4C7 (Ascites Free) - 3780594 3780594
      Anti-Laminin alpha5, clone 4C7 (Ascites Free) - 3783232 3783232
      Anti-Laminin alpha5, clone 4C7 (Ascites Free) - 4072180 4072180
      Anti-Laminin alpha5, clone 4C7 (Ascites Free) - 4154539 4154539
      Anti-Laminin alpha5, clone 4C7 (Ascites Free) - 4239686 4239686
      Anti-Laminin alpha5, clone 4C7 (Ascites Free) - 4309188 4309188
      Anti-Laminin alpha5, clone 4C7 (Ascites Free) - 4376187 4376187
      Anti-Laminin alpha5, clone 4C7 (Ascites Free) - 4388649 4388649
      Anti-Laminin alpha5, clone 4C7 (Ascites Free) Monoclonal Antibody Q2889123
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