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ABN1696 Anti-Harmonin (Ush1C)

Anti-Harmonin (Ush1C), Cat. No. ABN1696, is a highly specific rabbit polyclonal antibody that targets Harmonin and has been tested in Immunofluorescence and Western Blotting.

Anti-Harmonin (Ush1C) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Ush1c UniProt Number: Q9ES64
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ABN1696
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, IFRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1696
      DescriptionAnti-Harmonin (Ush1C)
      Alternate Names
      • Harmonin
      • PDZ domain-containing protein
      • Usher syndrome type-1C protein homolog
      Background InformationHarmonin (UniProt: Q9ES64; also known as PDZ domain-containing protein, Usher syndrome type-1C protein homolog) is encoded by the Ush1c gene (Gene ID 72088) in murine species. Harmonin is an anchoring/scaffolding protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. It is required for normal development and maintenance of cochlear hair cell bundles. Harmonin is reported to colocalize with F-actin and is detected at the tip of cochlear hair cell stereocilia. It is also enriched in microvilli of the intestinal brush border where as part of the intermicrovillar adhesion complex it plays a role in brush border differentiation, controlling microvilli organization and length. Harmonin contains PDZ domains, a coiled-coil region with a bipartite nuclear localization signal and a PEST degradation sequence. Defects in this gene are known to cause Usher syndrome type 1C and non-syndromic sensorineural deafness. Usher syndrome is characterized by profound hearing loss, vestibular dysfunction, and visual impairment.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in PBS without azide with 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Harmonin (Ush1C), Cat. No. ABN1696, is a highly specific rabbit polyclonal antibody that targets Harmonin and has been tested in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesImmunofluorescence Analysis: A 1-2 µg/mL from a representative lot immunostained connectting cilium of photoreceptor cells in frozen mouse retina tissue postfixed with paraformaldehyde (Courtesy of Dr. Tiansen Li at NIH).

      Western Blotting Analysis: A 1-2 µg/mL from a representative lot detected harmonin (Ush1C) in mouse retina tissue lysate (Courtesy of Dr. Tiansen Li at NIH).
      Biological Information
      ImmunogenHis-tagged recombinant mouse harmonin (Ush1C) fragment corresponding to the region N-terminal to the second PDZ domain.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody targets the region N-terminal to the second PDZ domain present in all three murine harmonin (Ush1C) spliced isoforms reported by UniProt (Q9ES64).
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ush1c
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~62 kDa observed. 68.19/96.50/102.3 kDa (mouse isoform a1/b2/b3; UniProt Q9ES64) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse kidney tissue lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected harmonin (Ush1C) in 10 µg of mouse kidney tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1696 04054839135286