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ABE1932 Anti-IRX-5

This rabbit polyclonal Anti-IRX-5 Antibody, Cat. No. ABE1932 detects levels of IRX-5, and has been published and validated for use in Western Blotting.

Anti-IRX-5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Irx5, Irxb2 UniProt Number: Q9JKQ4
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ABE1932
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWBRbUnpurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1932
      DescriptionAnti-IRX-5
      Alternate Names
      • Iroquois-class homeodomain protein IRX-5
      • Homeodomain protein IRXB2
      • Iroquois homeobox protein 5
      Background InformationIroquois-class homeodomain protein IRX-5 (UniProt Q9JKQ4; also known as Homeodomain protein IRXB2, Iroquois homeobox protein 5) is encoded by the Irx5 (also known as Irxb2) gene (Gene ID 54352) in murine species. Iroquois homeobox (Irx) genes encode a family of homeodomain transcription factors that are conserved from worms to vertebrates. Six IRX genes are located in two clusters in human (IRXA and IRXB) and mouse (IrxA and IrxB) genomes. Irx3 and Irx5 of the IrxB cluster are expressed in the developing mouse limb bud and are essential for proximal and anterior skeletal formation. Irx3 and Irx5 are required to establish early anteroposterior (AP) polarity and specify the anterior progenitor population. Individual Irx genes are also important for certain postnatal cardiac functions in the developing mouse heart, including cardiac repolarization (Irx5) and rapid ventricular conduction (Irx3). Postnatal deletion of Irx3 and Irx5 in the myocardium leads to prolongation of atrioventricular conduction, due in part to activation of expression of the Na(+) channel protein Nav1.5. However, combined postnatal loss of Irx3 and Irx5 results in a restoration of the repolarization gradient that is altered in Irx5 mutant hearts, suggesting that postnatal Irx3 activity can be repressed by Irx5 in mice.
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal antibody serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rabbit polyclonal Anti-IRX-5 Antibody, Cat. No. ABE1932 detects levels of IRX-5, and has been published and validated for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot was affinity purified and detected an increased IRX-5/IRXB2 expression in postnatal P10 heart tissue lysates from miR-1-2-knockout mice (Zhao, Y., et al. (2007). Cell. 129(2):303-317).
      Biological Information
      ImmunogenHis-tagged recombinant murine IRX-5/IRXB2 C-terminal fragment GST fusion protein (Chen, C.W., et al. (2005). Dev. Biol. 287(1):48-60).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antiserum targets an internal region within the C-terminal half of murine IRX-5/IRXB2.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Irx5
      • Irxb2
      Purification MethodUnpurified.
      UniProt Number
      Molecular Weight~54 kDa observed. 50.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in postnatal mouse heart tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antiserum detected IRX-5/IRXB2 in 10 µg of postnatal P10 mouse heart tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1932 04054839034633