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ABD119 Anti-TLE1 Antibody

This Anti-TLE1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of TLE1.

Anti-TLE1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Histone Modifying Proteins Gene Symbol: TLE1 UniProt Number: Q04724
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ABD119
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABD119
      DescriptionAnti-TLE1 Antibody
      Alternate Names
      • Transducin-like enhancer protein 1
      • E(Sp1) homolog
      • Enhancer of split groucho-like protein 1
      • ESG1
      Background InformationTransducin-like enhancer protein 1 (UniProt Q04724; also known as E(Sp1) homolog, Enhancer of split groucho-like protein 1, ESG1) is encoded by the TLE1 gene (Gene ID 7088) in human. TLE proteins interact with HDACs as well as deacetylated histone tails and play an important role in transcription regulation in several signaling pathways, including Ras, Notch, and Wnt. TLE proteins contain a conserved N-terminal glutamine-rich (Q) domain (a.a. 1-131 of TLE1) that mediates TLEs tetramerization as well as TCF/LEF proteins binding, and is essential for chromatin oligomerization. This is followed by a variable central domain sub-divided into a glycine-prolin-rich (GP) region (a.a. 132-199 of TLE1), the CcN (protein kinase CK2/cell cycle-dependent kinase 2/nuclear localization sequence) domain (a.a. 200-268), a serine-proline-rich (SP) region (a.a. 200-268 and 269-450 of TLE1), and a C-terminal WD40 domain with 6 WD repeats (a.a. 470-767 of TLE1) that interact with multiple transcription factors. The GP region binds directly to HDAC1, the CcN region contains consensus CK2 and Cdc2 phosphorylation sites that are modified in a cell cycle-dependent manner, the SP region is phosphorylated by the Ser/Thr kinases HIPK2 and MAPK, which appear to negatively regulate transcription repression activity, and may also interact with basic helix-loop-helix transcription factors. The C-terminal WD40 domain is essential for chromatin condensation and, together with SP, interacts with bHLH proteins.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-TLE1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of TLE1.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1 µg/mL from a representative lot detected TLE1 in HeLa cells and H9 human stem cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a sequence within the CcN domain of human TLE1.
      EpitopeCcN domain.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody targets the CcN (protein kinase CK2/cell cycle-dependent kinase 2/nuclear localization sequence) domain of TLE1 and stained cell nuclei by fluorescent immunocytochemistry.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TLE1
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~90 kDa observed. 83.20 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in H9 cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antiobdy detected TLE1 in 10 µg of H9 human stem cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABD119 04055977352832