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AB15610-I Anti-Prohormone Convertase PC2 Antibody

Anti-Prohormone Convertase PC2 Antibody is an antibody against Prohormone Convertase PC2 for use in Western Blotting, Immunohistochemistry.

Anti-Prohormone Convertase PC2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Hormones & Receptors Gene Symbol: PCSK2, NEC2 UniProt Number: P16519
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AB15610-I
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB15610-I
      ReplacesAB15610
      DescriptionAnti-Prohormone Convertase PC2 Antibody
      Alternate Names
      • KEX2-like endoprotease 2
      • NEC 2
      • PC2
      • Prohormone convertase 2
      • Proprotein convertase 2
      Background InformationNeuroendocrine convertase 2 (EC 3.4.21.94; UniProt P16519; also known as KEX2-like endoprotease 2, NEC 2, PC2, Prohormone convertase 2, Proprotein convertase 2) is encoded by the PCSK2 (also known as NEC2) gene (Gene ID 5126) in human. Neuroendocrine convertase 2/PC2 belongs to the family of subtilisin-related proteolytic enzymes involved in the proteolytic cleavages of peptide hormone precursors. For example, during the multistep processing of the peptide precursor proopiomelanocortin (POMC), PC2 catalyzes the cleavage of adrenocorticotropic hormone 1–39 (ACTH) into adrenocorticotropic hormone 1–14, which is then further processed to produce α-melanocyte-stimulating hormone (α-MSH). PC2 is produced initially in a prepro-form with a signal peptide (a.a. 1-25) and a propeptide (a.a. 26-109) sequence, the removal of which yields the mature 529-amino acid enzyme (a.a. 110-638). The neuroendocrine protein 7B2 blocks proPC2 from forming unactivatable aggregates and is absolutely required for processing proCP2 into active enzyme. Newly synthesized proPC2 is N-glycosylated in the ER and undergoes rate-limiting folding process before it can bind 7B2 and be transported to the TGN/ secretory granules for maturation by sulfation and proteolytic cleavage.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Prohormone Convertase PC2 Antibody is an antibody against Prohormone Convertase PC2 for use in Western Blotting, Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: An 1:1,000 dilution from a representative lot detected Prohormone Convertase PC2 in human cerebral cortex, pancreas and testis tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human mature PC2 N-terminal fragment.
      EpitopeNear the N-terminus of mature PC2.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects the ~68 kDa secreted mature PC2 in mouse cerebellum lysate by Western blotting. Expected to recognize also the Prepro- and pre-forms of all three spliced human isoforms reported by UniProt (P16519).
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteMouse, Human. Predicted to react with Porcine, Rhesus Macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PCSK2
      • NEC2
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~68 kDa calculated. Target band appears larger than the calculated molecular weight of the mature form (58.28 kDa/mouse & 58.00 kDa/human) due to posttranslational glycosylation and sulfation. 67.97/63.72/68.12 kDa (human isoform 1/2/3 proforms), 70.57/66.31/68.58 kDa (human isoform 1/2/3 prepro-forms) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse cerebellum tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Prohormone Convertase PC2 in 10 µg of mouse cerebellum tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB15610-I 04055977329391